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有读书笔记有附件Plant DELLAs Restrain Growth and Promote Survival of Adversity by Reducing the Levels of Reactive Oxygen Species

1 cflikunlun 添加于 2010-4-19 21:40 | 2197 次阅读 | 0 个评论
  •  作 者

    ACHARD P, RENOU J, BERTHOME R, HARBERD N, GENSCHIK P
  •  摘 要

    Plant growth is adaptively modulated in response to environmental change. The phytohormone gibberellin (GA) promotes growth by stimulating destruction of the nuclear growth-repressing DELLA proteins [1–7], thus providing a mechanism for environmentally responsive growth regulation [8, 9]. Furthermore, DELLAs promote survival of adverse environments [8]. However, the relationship between these survival and growth-regulatory mechanisms was previously unknown. Here, we show that both mechanisms are dependent upon control of the accumulation of reactive oxygen species (ROS). ROS are small molecules generated during development and in response to stress that play diverse roles as eukaryotic intracellular second messengers [10]. We show that Arabidopsis DELLAs cause ROS levels to remain low after either biotic or abiotic stress, thus delaying cell death and promoting tolerance. In essence, stressinduced DELLA accumulation elevates the expression of genes encoding ROS-detoxification enzymes, thus reducing ROS levels. In accord with recent demonstrations that ROS control root cell expansion [11, 12], we also show that DELLAs regulate root-hair growth via a ROS-dependent mechanism. We therefore propose that environmental variability regulates DELLA activity [8] and that DELLAs in turn couple the downstream regulation of plant growth and stress tolerance through modulation of ROS levels.
  •  详细资料

    • 文献种类: Journal Article
    • 期刊名称: Current Biology
    • 期刊缩写: Current Biology
    • 期卷页: 2008  18 9 656-660
    • ISBN: 0960-9822
  • 学科领域 生物医药 » 生物学

  • 相关链接 DOI URL 

  •  cflikunlun 的文献笔记  订阅

    DELLA提高抗逆性

    【原创】首先发现了DELLA在对植物生长发育的影响和耐盐性成相关性(相关系数),推测可能有共同的调节机制;为了研究这个机制,用GA突变体和DELLA突变体做了转录组分析,发现2.2%(540条)的基因在盐胁迫下的mRNA水平是依赖于DELLA元件的,又将这些基因与芯片数据(受盐,干旱,病原体影响)比对,发现其中126条基因是是和氧化胁迫相关的,在DELLA积累的植株中抗氧化系统的成分基因上调,并且受DELLA调控的基因大部分都是受到胁迫后呈现早期诱导或抑制表达,因此stress-induced DELLA accumulation activates a complex genetic regulation network to control in part the amount of ROS.
    DELLA通过ROS机制(影响cell expation) 来调控根毛生长(GA调控)
    这篇文章主要是将DELLA与盐胁迫通过ROS这个共同机制联系,但是GA和非生物胁迫的关系并未深入研究!
    另外,ion leakage 作为cell death的测定指标;
    另外,学到了几种染料:
    H2DCFDA—— a ROS-sensitive dye
    DAB, a hydrogen peroxide [H2O2]-sensitive dye
    DPI, inhibitor of flavoprotein activity such as NADPH oxidases
    NBT, a superoxide [O22]-sensitive dye
    PI—— a nucleic-acid stain that can only penetrate cells with damaged or leaking cell membranes——investigate cell death

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