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Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

热1 ingrid_86 添加于 2009-7-27 18:45 | 1920 次阅读 | 2 个评论

作者
Geurts AM, Cost GJ, Freyvert Y, Zeitler B, Miller JC, Choi VM, Jenkins SS, Wood A, Cui X, Meng X, Vincent A, Lam S, Michalkiewicz M, Schilling R, Foeckler J, Kalloway S, Weiler H, Menoret S, Anegon I, Davis GD, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jacob HJ, Buelow R
摘要
The toolbox of rat genetics currently lacks the ability to introduce site-directed, heritable mutations into the genome to create knockout animals. By using engineered zinc-finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one-cell rat embryo leads to a high frequency of animals carrying 25 to 100% disruption at the target locus. These mutations are faithfully and efficiently transmitted through the germline. Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.
详细资料
- 文献种类:期刊
- 期刊名称: Science (New York, N.Y.)
- 期刊缩写: Science
- 期卷页: 2009年第325卷第5939期 433页
- 地址: Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 52336, USA
- ISBN: 1095-9203
标签

Knockout
相关链接 DOI URL