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Direct Microfabrication of Topographical and Chemical Cues for the Guided Growth of Neural Cell Networks on Polyamidoamine Hydrogels

阿土狗添加于 2010-6-11 08:40 | 1180 次阅读 | 0 个评论

作者
Dos Reis G, Fenili F, Gianfelice A, Bongiorno G, Marchesi D, Scopelliti PE, Borgonovo A, Podestà A, Indrieri M, Ranucci E, Ferruti P, Lenardi C, Milani P
摘要
Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri-cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell-based arrays, stem cell therapy and cell-based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub-micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e-beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti-fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E-beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells.
详细资料
- 文献种类:期刊
- 期刊名称: Macromolecular Bioscience
- 期刊缩写: Macromol. Biosci.
- 期卷页: 2010年 n/a-n/a页
- ISBN: 1616-5187
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