Promoter of a cotton fibre MYB gene functional in trichomes of Arabidopsis and glandular trichomes of tobacco
butterfly09 添加于 2010-6-19 17:02
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作 者
Shangguan X-X, Xu B, Yu Z-X, Wang L-J, Chen X-Y 摘 要
Cotton fibres are unicellular seed trichomes. Our pre-
vious study suggested that the cotton R2R3 MYB
transcript factor GaMYB2 is a functional homologue of
the Arabidopsis trichome regulator GLABRA1 (GL1).
Here, the GaMYB2 promoter activity is reported in
cotton (Gossypium hirsutum), tobacco (Nicotiana taba-
cum), and Arabidopsis plants. A 2062 bp promoter of
GaMYB2 was isolated from G. arboreum, and fused to
a b-glucuronidase (GUS) reporter gene. In cotton, the
GaMYB2 promoter exhibited activities in developing
fibre cells and trichomes of other aerial organs, in-
cluding leaves, stems and bracts. In Arabidopsis the
promoter was specific to trichomes. Different from
Arabidopsis and cotton that have unicellular non-
glandular simple trichomes, tobacco plants contain
more than one type of trichome, including multicellular
simple and glandular secreting trichomes (GSTs).
Interestingly, in tobacco plants the GaMYB2 promoter
directed GUS expression exclusively in glandular cells
of GSTs. A series of 5#-deletions revealed that a 360 bp
fragment upstream to the translation initiation codon
was sufficient to drive gene expression. A putative cis-
element of the T/G-box was located at -233 to -214;
a yeast one-hybrid assay showed that Arabidopsis
bHLH protein GLABRA3 (GL3), also a trichome regula-
tor, and GhDEL65, a GL3-like cotton protein, had high
binding activities to the T/G-box motif. Overexpression
of GL3 or GhDEL65 enhanced the GaMYB2 promoter
activity in transgenic Arabidopsis plants. A compari-
son of GaMYB2 promoter specificities in trichomes of
different plant species with different types of trichomes
provides a tool for further dissection of plant trichome
structure and development.-
详细资料
- 文献种类:期刊
- 期刊名称: Journal of Experimental Botany
- 期刊缩写: Journal of Experimental Botany
- 期卷页: 2008年 第59卷 第13期 3533-3542页
- ISBN: 0022-0957
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