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Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment

butterfly09 添加于 2010-8-23 00:16 | 1731 次阅读 | 0 个评论

作者
Ying Ge, Yong Li, Yan-Ming Zhu, Xi Bai, De-Kang Lv, Dianjing Guo, Wei Ji, Hua Cai
摘要
Background Plant roots are the primary site of perception and injury for saline-alkaline stress. The current knowledge of saline-alkaline stress transcriptome is mostly focused on saline (NaCl) stress and only limited information on alkaline (NaHCO3) stress is available. Results Using Affymetrix Soybean GeneChip, we conducted transcriptional profiling on Glycine soja roots subjected to 50mmol/L NaHCO3 treatment. In a total of 7088 probe sets, 3307 were up-regulated and 5720 were down-regulated at various time points. The number of significantly stress regulated genes increased dramatically after 3h stress treatment and peaked at 6h. GO enrichment test revealed that most of the differentially expressed genes were involved in signal transduction, energy, transcription, secondary metabolism, transporter, disease and defence response. We also detected 11 microRNAs regulated by NaHCO3 stress. Conclusions This is the first comprehensive wild soybean root transcriptome analysis under alkaline stress. These analyses have identified an inventory of genes with altered expression regulated by alkaline stress. The data extend the current understanding of wild soybean alkali stress response by providing a set of robustly selected, differentially expressed genes for further investigation.
详细资料
- 文献种类:期刊
- 期刊名称: BMC Plant Biology
- 期卷页: 2010年第10卷 153页
- 日期: 2010-7-26
标签

alkaline NaHCO3 glycine soja
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