Expressed sequence tag (EST) projects have generated a
vast amount of publicly available sequence data from
plant species; these data can be mined for simple
sequence repeats (SSRs). These SSRs are useful as
molecular markers because their development is inexpensive,
they represent transcribed genes and a putative
function can often be deduced by a homology search.
Because they are derived from transcripts, they are
useful for assaying the functional diversity in natural
populations or germplasm collections. These markers
are valuable because of their higher level of transferability
to related species, and they can often be used as
anchor markers for comparative mapping and evolutionary
studies. They have been developed and
mapped in several crop species and could prove useful
for marker-assisted selection, especially when the
markers reside in the genes responsible for a phenotypic
trait. Applications and potential uses of EST-SSRs in
plant genetics and breeding are discussed.