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Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples

francisliu 添加于 2010-11-29 21:37 | 2202 次阅读 | 0 个评论

作者
Berlemont R, Delsaute M, Pipers D, D'Amico S, Feller G, Galleni M, Power P
摘要
In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 °C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.
详细资料
- 文献种类:期刊
- 期刊名称: The ISME Journal
- 期刊缩写: ISME J
- 期卷页: 2009年第3卷第9期 1070-1081页
- ISBN: 1751-7362
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the important information of this article

1,RBcel1(a cold-adapted protein) that hydrolyzes only carboxymethyl cellulose.

2,RBcel1 displayed an endoglucanase activity,producing cellobiose and cellotriose,using carboxymethyl cellulose as a substrate.

3,Moreover,the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased(18% of activity at 10℃).

4,It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate.

5,By a combination of bioinformatics and enzyme analysis,the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria.

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