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Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors

qinkunhua 添加于 2011-2-20 17:47 | 1121 次阅读 | 0 个评论

作者
Treisman R
摘要
Transient transcriptional activation of the c-fos gene following serum stimulation of susceptible cells requires a conserved DNA element located 300 bp 5' to the mRNA cap site. A DNA-binding gel electrophoresis assay was used to detect a protein(s) in HeLa cell nuclear extracts that specifically binds to the 5' activating element. The protein recognizes a region of dyad symmetry within the 5' activating element, defined by binding competition, dimethylsulphate (DMS) interference and DNAase I and DMS protection studies. A single 22 bp synthetic copy of the dyad symmetry element will both compete efficiently for protein binding and restore serum regulation to c-fosH genes that lack the 5' activating element.
详细资料
- 文献种类:期刊
- 期刊名称: Cell
- 期刊缩写: Cell
- 期卷页: 1986年第46卷第4期 567-574页
- ISBN: 0092-8674
- 备注:PMID:3524858
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