新科学想法 › 文献管理 › 浏览文献

Experimental illumination of a fitness landscape

王关中添加于 2011-4-19 16:07 | 1542 次阅读 | 0 个评论

作者
Hietpas RT, Jensen JD, Bolon DNA
摘要
The genes of all organisms have been shaped by selective pressures. The relationship between gene sequence and fitness has tremendous implications for understanding both evolutionary processes and functional constraints on the encoded proteins. Here, we have exploited deep sequencing technology to experimentally determine the fitness of all possible individual point mutants under controlled conditions for a nine-amino acid region of Hsp90. Over the past five decades, limited glimpses into the relationship between gene sequence and function have sparked a long debate regarding the distribution, relative proportion, and evolutionary significance of deleterious, neutral, and advantageous mutations. Our systematic experimental measurement of fitness effects of Hsp90 mutants in yeast, evaluated in the light of existing population genetic theory, are remarkably consistent with a nearly neutral model of molecular evolution.
详细资料
- 文献种类:期刊
- 期刊名称: Proceedings of the National Academy of Sciences
- 期刊缩写: Proceedings of the National Academy of Sciences
- ISBN: 0027-8424
标签

new_method
相关链接 DOI URL

2 人收藏