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MicroRNA-22 Regulates Hypoxia Signaling in Colon Cancer Cells

dangdang 添加于 2011-11-24 21:28 | 1554 次阅读 | 0 个评论

作者
Yamakuchi M, Yagi S, Ito T, Lowenstein CJ, Emanueli C
摘要
MicroRNAs (MiRNAs) are short, non-coding RNA that regulate a variety of cellular functions by suppressing target protein expression. We hypothesized that a set of microRNA regulate tumor responses to hypoxia by inhibiting components of the hypoxia signaling pathway. We found that miR-22 expression in human colon cancer is lower than in normal colon tissue. We also found that miR-22 controls hypoxia inducible factor 1α (HIF-1α) expression in the HCT116 colon cancer cell line. Over-expression of miR-22 inhibits HIF-1α expression, repressing vascular endothelial growth factor (VEGF) production during hypoxia. Conversely, knockdown of endogenous miR-22 enhances hypoxia induced expression of HIF-1α and VEGF. The conditioned media from cells over-expressing miR-22 contain less VEGF protein than control cells, and also induce less endothelial cell growth and invasion, suggesting miR-22 in adjacent cells influences endothelial cell function. Taken together, our data suggest that miR-22 might have an anti-angiogenic effect in colon cancer.
详细资料
- 文献种类:期刊
- 期刊名称: PLoS ONE
- 期刊缩写: PLoS ONE
- 期卷页: 2011年第6卷第5期 e20291页
- ISBN: 1932-6203
标签

miR22
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