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miRNeye: a microRNA expression atlas of the mouse eye

drjiangbo 添加于 2011-12-27 00:26 | 1202 次阅读 | 0 个评论

作者
Karali M, Peluso I, Gennarino VA, Bilio M, Verde R, Lago G, Dolle P, Banfi S
摘要
BACKGROUND: MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. RESULTS: In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. CONCLUSIONS: This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures.
详细资料
- 关键词: Animals; Ciliary Body/cytology/metabolism; Cornea/cytology/metabolism; *Databases, Genetic; Eye/cytology/growth & development/*metabolism; *Gene Expression Profiling; Gene Expression Regulation, Developmental; In Situ Hybridization; Internet; Iris/cytology/metabolism; Lens, Crystalline/cytology/metabolism; Mice; Mice, Inbred C57BL; MicroRNAs/*genetics/metabolism; Organ Specificity/genetics; Retinal Pigment Epithelium/cytology/metabolism; Time Factors
- 文献种类:期刊
- 期刊名称: BMC Genomics
- 期刊缩写: BMC Genomics
- 期卷页: 2010年第11卷 715页
- 地址: Telethon Institute for Genetics and Medicine, Via P. Castellino 111, Naples, Italy
- ISBN: 1471-2164
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