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[Application of liquid chromatography coupled with mass spectrometry (LC/MS) to determine antidepressants in blood samples]

jiangfyh 添加于 2009-9-10 14:05 | 1829 次阅读 | 0 个评论

作者
Pufal E, Sykutera M
摘要
A simultaneous determination of twelve antidepressant drugs (amitriptyline, chlorpromazine, clomipramine, doxepin, fluoxetine, levomepromazine, maprotiline, paroxetine, perazine, promazine, sertraline, thioridazine) in blood samples was developed using high-performance liquid chromatography with mass spectrometry (LC/MS). Blood samples were extracted with acetonitrile and separated by HPLC on a reversed phase C18 column with a mobile phase consisting of acetonitrile -0.1% trifluoroacetic acid (50:50 (v/v) at a flow-rate of 0.4 ml/min. The analytes were determined using electrospray ionization in a single quadrupole mass spectrometer. LC-ESI-MS was performed in the selected-ion monitoring (SIM) mode using target ions. The validated method was successfully used to analyze antidepressants in blood samples collected from four victims of suicide by hanging.
详细资料
- 关键词: Antidepressive Agents, Tricyclic/*blood; Blood Chemical Analysis; Chromatography, High Pressure Liquid/methods; Forensic Medicine/*methods; Gas Chromatography-Mass Spectrometry/*methods; Humans; Indicators and Reagents/chemistry; Poland; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization/*methods
- 文献种类:期刊
- 期刊名称: Archiwum Medycyny Sadowej i Kryminologii
- 期刊缩写: Arch Med Sadowej Kryminol
- 期卷页: 2008年第58卷第4期 171-176页
- 地址: Katedry Medycyny Sadowej UMK w Toruniu, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy
- ISBN: 0324-8267
- 备注:PMID:19441687
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