新科学想法 › 文献管理 › 浏览文献

The role of the 5-hydroxytryptamine pathway in reflux-induced esophageal mucosal injury in rats

icecream 添加于 2012-11-3 10:32 | 1068 次阅读 | 0 个评论

作者
Yang L, Cai H, Tou J, Gu W, Shu X, Zhang T, Yang X, Shen Z, Jiang M
摘要
ABSTRACT: BACKGROUND: Dysfunction of the 5-hydroxytryptamine (5-HT) signaling pathway can lead to gastrointestinal motility and secretion abnormalities and to visceral hypersensitivity. The aim of this study is to investigate the role of 5-HT in reflux-induced esophageal mucosal injury. METHODS: Fifty 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a gastroesophageal reflux (GER) model group (30 rats) and a sham surgery control group (20 rats). Four weeks after surgery, the esophageal mucosa was collected for histological evaluation, 5-HT concentrations, and 5-HT selective reuptake transporter (SERT) mRNA and 5-HT4 receptor (5-HT4R) protein expressions. RESULTS: Twenty-seven rats in the GER model group survived, and three rats died. Histologically, in the GER model group, 20 rats had reflux esophagitis (RE group), and 7 rats had non-erosive reflux disease (NERD group). The 5-HT levels in the esophageal tissue from the RE group were significantly higher than those from the control and NERD groups. Both the RE and NERD groups showed significant increases in SERT mRNA expression of the esophageal mucosa than that of the controls, and the SERT mRNA level in the RE group was significantly higher than that in the NERD group. The 5-HT4R protein level of the esophageal mucosa in the RE group was significantly lower than that in the controls and the NERD group. CONCLUSIONS: We conclude that a 5-HT signaling pathway disorder could be a major factor in the pathogenesis of GER and RE.
详细资料
- 文献种类:期刊
- 期刊名称: World Journal of Surgical Oncology
- 期刊缩写: World J Surg Oncol
- 期卷页: 2012年第10卷第1期 219页
- ISBN: 1477-7819
相关链接 DOI URL