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DNA damage-induced CHK1 autophosphorylation at Ser296 is regulated by an intramolecular mechanism

fanfan 添加于 2012-11-12 05:21 | 665 次阅读 | 0 个评论

作者
Okita N, Minato S, Ohmi E, Tanuma S-I, Higami Y
摘要
CHK1 regulates the DNA damage-induced checkpoint involving an ATR- or ATM- dependent pathway. In this paper, we focused on the autophosphorylation of Ser296, one of the DNA damage-induced phosphorylation sites. First, we demonstrated that the Ser296 autophosphorylation of CHK1 is mainly regulated by an intramolecular mechanism in response to DNA damage. In examining the relationship between Ser296 and Ser317/Ser345, the other ATR dependent phosphorylation sites, we found that the Ser296 cis-autophosphorylation was dependent on both Ser317 and Ser345 phosphorylation. Our findings suggest that CHK1 mediates cell cycle checkpoint signals by both cis-autophosphorylation and trans-phosphorylation of downstream factors. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: CHK1phosphorylatesCHK1 by protein kinase assay (View Interaction: 1, 2, 3).
详细资料
- 文献种类:期刊
- 期刊名称: FEBS Letters
- 期刊缩写: FEBS Lett
- 期卷页: 2012年
- 地址: Department of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-0022, Japan. Electronic address: nokita7@rs.noda.tus.ac.jp
- ISBN: 0014-5793
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