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Genetic variants in miR-146a, miR-149, miR-196a2, miR-499 and their influence on relative expression in lung cancers

icecream 添加于 2012-12-10 20:43 | 1496 次阅读 | 0 个评论

作者
Vinci S, Gelmini S, Pratesi N, Conti S, Malentacchi F, Simi L, Pazzagli M, Orlando C
摘要
BACKGROUND: The presence of sequence variants in miRNA genes may influence their processing, expression and binding to target mRNAs. Since single miRNA can have a large number of potential mRNA targets, even minor variations in its expression can have influences on hundreds of putative mRNAs. METHODS: Here, we evaluated 101 paired samples (cancer and normal tissues) from non-small cell lung carcinoma (NSCLC) patients to study the genotype distribution of single nucleotide polymorphisms (SNPs) in miR-146a (rs2910164 C-G), miR-149 (rs2292832 C-T), miR-196a2 (rs11614913 C-T) and miR-499 (rs3746444 G-A) and their influence on the expression of respective miRNAs. RESULTS: Relative expression of miR-146a, miR-149 and miR-499 were comparable in NSCLC and in paired control tissues. On the contrary, we clearly detected a significant increase (p<0.001) of miR-196a2 expression in NSCLC. In particular we found a significant association between miR-196a2 CC genotype and high expression, whereas TT geno-type showed a very low expression in comparison to both CT (p<0.005) and CC patients (p<0.01). We did not find any association between miR-149, miR-196a2 and miR-499 genotype and risk of NSCLC. Conversely, CG genotype of miR-146a appeared associated to an increased risk for NSCLC (p=0.042 and 1.77 OR). CONCLUSIONS: Our results seem to demonstrate that sequence variants of miR-196a2 can have an influence on its expression, while miR-146a can have a role in increasing the risk of NSCLC.
详细资料
- 关键词: Adult; Aged; Aged, 80 and over; Alleles; Carcinoma, Non-Small-Cell Lung/*genetics/metabolism; Case-Control Studies; Female; Gene Frequency; Genotype; Humans; Lung Neoplasms/*genetics/metabolism; Male; MicroRNAs/genetics/*metabolism; Middle Aged; Polymorphism, Single Nucleotide; Real-Time Polymerase Chain Reaction
- 文献种类:期刊
- 期刊名称: Clinical Chemistry and Laboratory Medicine : CCLM / FESCC
- 期刊缩写: Clin Chem Lab Med
- 期卷页: 2011年第49卷第12期 2073-2080页
- 地址: Clinical Biochemistry, Department of Clinical Physiopathology, University of Florence, Florence, Italy
- ISBN: 1434-6621
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