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Three-dimensional folding and functional organization principles of the Drosophila genome

聚焦生物添加于 2012-12-26 20:14 | 2118 次阅读 | 0 个评论

作者
Sexton T, Yaffe E, Kenigsberg E, Bantignies F, Leblanc B, Hoichman M, Parrinello H, Tanay A, Cavalli G
摘要
Chromosomes are the physical realization of genetic information and thus form the basis for its readout and propagation. Here we present a high-resolution chromosomal contact map derived from a modified genome-wide chromosome conformation capture approach applied to Drosophila embryonic nuclei. The data show that the entire genome is linearly partitioned into well-demarcated physical domains that overlap extensively with active and repressive epigenetic marks. Chromosomal contacts are hierarchically organized between domains. Global modeling of contact density and clustering of domains show that inactive domains are condensed and confined to their chromosomal territories, whereas active domains reach out of the territory to form remote intra- and interchromosomal contacts. Moreover, we systematically identify specific long-range intrachromosomal contacts between Polycomb-repressed domains. Together, these observations allow for quantitative prediction of the Drosophila chromosomal contact map, laying the foundation for detailed studies of chromosome structure and function in a genetically tractable system.
详细资料
- 关键词: Animals; Cell Nucleus/genetics; Chromosomes, Insect; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology/embryology/*genetics; *Genome, Insect; Models, Statistical; Polycomb Repressive Complex 1
- 文献种类:期刊
- 期刊名称: Cell
- 期刊缩写: Cell
- 期卷页: 2012年第148卷第3期 458-472页
- 地址: Institut de Genetique Humaine, UPR 1142, CNRS, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
- ISBN: 0092-8674
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附件
Three-dimensional folding and functional organization principles of the Drosophila genome
聚焦生物的文献笔记订阅

Sexton等人提出了一个高分辨率果蝇核染色质图谱,为理解后生动物核组织提供了新的基础。