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piRNAs initiate an epigenetic memory of nonself RNA in the C. elegans germline

聚焦生物添加于 2012-12-26 21:54 | 2896 次阅读 | 0 个评论

作者
Shirayama M, Seth M, Lee H-C, Gu W, Ishidate T, Conte DJ, Mello CC
摘要
Organisms employ a fascinating array of strategies to silence invasive nucleic acids such as transposons and viruses. Although evidence exists for several pathways that detect foreign sequences, including pathways that sense copy number, unpaired DNA, or aberrant RNA (e.g., dsRNA), in many cases, the mechanisms used to distinguish \"self\" from \"nonself\" nucleic acids remain mysterious. Here, we describe an RNA-induced epigenetic silencing pathway that permanently silences single-copy transgenes. We show that the Piwi Argonaute PRG-1 and its genomically encoded piRNA cofactors initiate permanent silencing, and maintenance depends on chromatin factors and the WAGO Argonaute pathway. Our findings support a model in which PRG-1 scans for foreign sequences and two other Argonaute pathways serve as epigenetic memories of \"self\" and \"nonself\" RNAs. These findings suggest how organisms can utilize RNAi-related mechanisms to detect foreign sequences not by any molecular signature, but by comparing the foreign sequence to a memory of previous gene expression.
详细资料
- 关键词: Animals; Argonaute Proteins/genetics/metabolism; Caenorhabditis elegans/*genetics/metabolism; Caenorhabditis elegans Proteins/genetics/metabolism; *Epigenomics; Gene Silencing; Germ Cells/metabolism; RNA Interference; RNA, Helminth/*metabolism; RNA, Small Interfering/*metabolism
- 文献种类:期刊
- 期刊名称: Cell
- 期刊缩写: Cell
- 期卷页: 2012年第150卷第1期 65-77页
- 地址: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
- ISBN: 0092-8674
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piRNAs initiate an epigenetic memory of nonself RNA in the C. elegans germline