Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase
北方的河 添加于 2013-3-4 15:21
| 1295 次阅读 | 0 个评论
作 者
Buganim Y, Faddah DA, Cheng AW, Itskovich E, Markoulaki S, Ganz K, Klemm SL, van Oudenaarden A, Jaenisch R 摘 要
During cellular reprogramming, only a small fraction of cells become induced pluripotent stem cells (iPSCs). Previous analyses of gene expression during reprogramming were based on populations of cells, impeding single-cell level identification of reprogramming events. We utilized two gene expression technologies to profile 48 genes in single cells at various stages during the reprogramming process. Analysis of early stages revealed considerable variation in gene expression between cells in contrast to late stages. Expression of Esrrb, Utf1, Lin28, and Dppa2 is a better predictor for cells to progress into iPSCs than expression of the previously suggested reprogramming markers Fbxo15, Fgf4, and Oct4. Stochastic gene expression early in reprogramming is followed by a late hierarchical phase with Sox2 being the upstream factor in a gene expression hierarchy. Finally, downstream factors derived from the late phase, which do not include Oct4, Sox2, Klf4, c-Myc, and Nanog, can activate the pluripotency circuitry.-
详细资料
- 关键词: Animals; Cell Line; Embryo, Mammalian/cytology; Embryonic Stem Cells; Fibroblasts/cytology/metabolism; Genetic Markers; Induced Pluripotent Stem Cells/cytology/*metabolism; Mice; Microfluidic Analytical Techniques; *Nuclear Reprogramming; SOXB1 Transcription Factors/metabolism; *Single-Cell Analysis; Transcription Factors/metabolism; *Transcriptome
- 文献种类:期刊
- 期刊名称: Cell
- 期刊缩写: Cell
- 期卷页: 2012年 第150卷 第6期 1209-1222页
- 地址: The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
- ISBN: 0092-8674
评论( 人)
相关链接
DOI URL
浙公网安备 33010202000686号 ( 浙ICP备09035230号-1 )
