新科学想法 › 文献管理 › 浏览文献

microRNAs Derived from Circulating Exosomes as Noninvasive Biomarkers for Screening and Diagnosing Lung Cancer

pandon2002 添加于 2013-8-19 00:32 | 1579 次阅读 | 0 个评论

作者
Cazzoli R, Buttitta F, Di Nicola M, Malatesta S, Marchetti A, Rom WN, Pass HI
摘要
INTRODUCTION:: Lung cancer is the highest cause of mortality among tumor pathologies worldwide. There are no validated techniques for an early detection of pulmonary cancer lesions other than low-dose helical computed tomography scan. Unfortunately, this method has some negative effects. Recent studies have laid the basis for development of exosomes-based techniques to screen/diagnose lung cancers. As the isolation of circulating exosomes is a minimally invasive procedure, this technique opens new possibilities for diagnostic applications. METHODS:: We used a first set of 30 plasma samples from as many patients, including 10 patients affected by lung adenocarcinomas, 10 with lung granulomas, and 10 healthy smokers matched for age and sex as negative controls. Wide-range microRNAs analysis (742 microRNAs) was performed by quantitative real time polymerase chain reaction. Data were compared on the basis of lesion characteristics, using WEKA software for statistics and modeling. Subsequently, selected microRNAs were evaluated on an independent larger group of samples (105 specimens: 50 lung adenocarcinomas, 30 lung granulomas, and 25 healthy smokers). RESULTS:: This analysis led to the selection of four microRNAs to perform a screening test (miR-378a, miR-379, miR-139-5p, and miR-200b-5p), useful to divide population into two groups: nodule (lung adenocarcinomas + carcinomas) and non-nodule (healthy former smokers). Six microRNAs (miR-151a-5p, miR-30a-3p, miR-200b-5p, miR-629, miR-100, and miR-154-3p) were selected for a second test on the nodule population to discriminate between lung adenocarcinoma and granuloma. CONCLUSIONS:: The screening test showed 97.5% sensitivity, 72.0% specificity, and area under the curve receiver operating characteristic of 90.8%. The diagnostic test had 96.0% sensitivity, 60.0% specificity, and area under the curve receiver operating characteristic of 76.0%. Further evaluation is needed to confirm the predictive power of these models on larger cohorts of samples.
详细资料
- 文献种类:期刊
- 期刊名称: Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer
- 期刊缩写: J Thorac Oncol
- 期卷页: 2013年第8卷第9期 1156-1162页
- 地址: *Center of Predictive Molecular Medicine, Center of Excellence on Aging, daggerOncological and Cardiovascular Molecular Medicine Unit, University-Foundation, Chieti, Italy; double daggerLaboratory of Biostatistics, Department of Biomedical Sciences, University "G. d\'Annunzio," Chieti, Italy; section signDivision of Pulmonary, Critical Care and Sleep, New York University School of Medicine, New York, New York; ||School of Medicine, Department of Environmental Medicine, New York University, Tuxedo Park, New York, and paragraph signDepartment of Cardiothoracic Surgery, New York University School of Medicine, New York, New York
- ISBN: 1556-0864
相关链接 DOI URL