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Development and immunological evaluation of HLA-specific chronic myeloid leukemia polyepitope vaccine in Chinese population

tomqiao99 添加于 2015-4-6 14:17 | 1435 次阅读 | 0 个评论

作者
Dong W, Zhang J, Shao N, Tian T, Li L, Jian J, Zang S, Ma D, Ji C
摘要
BACKGROUND: BCR/ABL and Wilms\' tumor 1 (WT1) are an ideal tumor associated antigens which can be used to develop a potential chronic myeloid leukemia (CML) dentritic cell (DC) vaccine. Here, we constructed a novel polyepitope vaccine which used recombinant lentiviral vector carrying BCR/ABL and WT1 genes, and determined the immunological effects of this vaccine in vitro. METHODS: The DC vaccine was constructed using lentiviral vector transduced DCs. T lymphocytes were stimulated with DC vaccine and then co-cultured in vitro with peripheral blood mononuclear cells (PBMCs) from CML or ALL patients, respectively. The cytotoxicity of proliferous cytotoxic T lymphocytes (CTLs) was determined by the LDH assay. The IFN-gamma production of CTLs was detected using ELISPOT assay. RESULTS: We constructed an lentiviral vector encoding 50 different epitopes from BCR/ABL and WT1 antigens, and transferred it into DCs to prepare the DC vaccine successfully. The in vivo stimulation of CTLs with this DC vaccine were proved to show strong cytotoxicity and produce high level of IFN-gamma. CONCLUSIONS: The novel recombinant lentiviral polyepitope DC vaccine is a promising candidate for clinical trials and may be an effective approach for CML immunotherapy.
详细资料
- 关键词: Adult; Aged; Antigens, Neoplasm/immunology; Asian Continental Ancestry Group; Cancer Vaccines/*immunology; Cells, Cultured; Dendritic Cells/*immunology; Epitopes/immunology; Female; Fusion Proteins, bcr-abl/*immunology; Genes, MHC Class I; Genes, MHC Class II; Genetic Vectors; HEK293 Cells; Humans; Immunotherapy; Interferon-gamma/immunology; Lentivirus; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*prevention & control; Leukocytes, Mononuclear/immunology; Male; Middle Aged; T-Lymphocytes, Cy ...
- 文献种类:期刊
- 期刊名称: Vaccine
- 期刊缩写: Vaccine
- 期卷页: 2014年第32卷第28期 3501-3508页
- 地址: Department of Hematology, Qilu Hospital, Shandong University, Jinan, China. Electronic address: jichunyan@sdu.edu.cn
- ISBN: 0264-410X
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