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Characterization of immune responses induced by inactivated, live attenuated and DNA vaccines against Japanese encephalitis virus in mice

tomqiao99 添加于 2015-4-6 14:19 | 1274 次阅读 | 0 个评论

作者
Li J, Chen H, Wu N, Fan D, Liang G, Gao N, An J
摘要
Vaccination is the most effective countermeasure for protecting individuals from Japanese encephalitis virus (JEV) infection. There are two types of JEV vaccines currently used in China: the Vero cell-derived inactivated vaccine and the live attenuated vaccine. In this study, we characterized the immune response and protective efficacy induced in mice by the inactivated vaccine, live attenuated vaccine and the DNA vaccine candidate pCAG-JME, which expresses JEV prM-E proteins. We found that the live attenuated vaccine conferred 100% protection and resulted in the generation of high levels of specific anti-JEV antibodies and cytokines. The pCAG-JME vaccine induced protective immunity as well as the live attenuated vaccine. Unexpectedly, immunization with the inactivated vaccine only induced a limited immune response and partial protection, which may be due to the decreased activity of dendritic cells and the expansion of CD4+CD25+Foxp3+ regulatory T cells observed in these mice. Altogether, our results suggest that the live attenuated vaccine is more effective in providing protection against JEV infection than the inactivated vaccine and that pCAG-JME will be a potential JEV vaccine candidate.
详细资料
- 关键词: Animals; Dendritic Cells/immunology; Encephalitis Virus, Japanese/immunology/*pathogenicity; Encephalitis, Japanese/immunology/*prevention & control; Female; Forkhead Transcription Factors/immunology; Interleukin-2 Receptor alpha Subunit/immunology; Japanese Encephalitis Vaccines/*immunology; Mice; Mice, Inbred BALB C; T-Lymphocytes/immunology; Vaccines, Attenuated/*immunology; Vaccines, DNA/*immunology; Vaccines, Inactivated/immunology; DNA vaccine; Dentritic cell; Inactivated vaccine; Japanese ...
- 文献种类:期刊
- 期刊名称: Vaccine
- 期刊缩写: Vaccine
- 期卷页: 2013年第31卷第38期 4136-4142页
- 地址: Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China
- ISBN: 0264-410X
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