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Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein

lixinnew027 添加于 2009-11-13 14:45 | 2317 次阅读 | 0 个评论

作者
Li GC, Li N, Zhang YH, Li X, Wang YG, Liu XY, Qian WB, Liu XC
摘要
Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development.
详细资料
- 关键词: Agglutinins/*diagnostic use/genetics; Cell Line, Tumor; Humans; Leukemia, Myeloid/diagnosis/*pathology; Mannose/*metabolism; Mannose-Binding Lectin/*diagnostic use; Pinellia/genetics; Receptors, Virus/genetics; Recombinant Fusion Proteins/diagnostic use
- 文献种类:期刊
- 期刊名称: International Journal of Hematology
- 期刊缩写: Int J Hematol
- 期卷页: 2009年第89卷第5期 611-617页
- 地址: Xin Yuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China
- ISBN: 1865-3774
- 备注:PMID:19377843
标签

leukemia mannose
相关链接 DOI URL
附件
Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein

lixinnew027 的文献笔记订阅

糖基化的改变可能是恶性肿瘤转化和癌症发生的一个重要标志，在本文中一种特异的甘露糖结合凝集素-掌叶半夏凝集素（PPA），与腺病毒-科萨奇病毒胞外段（sCAR）形成融合蛋白，通过sCAR-PPA融合蛋白可以提高急性髓性白血病细胞系Kasumi-1 和 HL-60 对腺病毒的感染率，提示AML细胞表面的甘露糖残基可以被PPA识别，然而，PPA没有增加腺病毒对KG-1细胞的感染率，说明AML细胞表型具有细胞特异性，并且sCAR-PPA融合蛋白也可以显著提高腺病毒对慢性髓系白血病细胞K562的感染效果。利用sCAR-PPA融合蛋白和AD-GFP可以为研究白血病细胞表面甘露糖残基的作用以及在白血病发生发展中的作用。在本研究中。首次发现掌叶半夏凝集素（PPA）可以识别白血病细胞表面的甘露糖残基。

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