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A protocol for unraveling gene regulatory networks

热1 wangjianwu 添加于 2009-11-16 21:29 | 1925 次阅读 | 0 个评论

作者
Materna SC, Oliveri P
摘要
Regulatory genes form large networks that are fundamental to the developmental program. The protocol presented here describes a general approach to assemble maps of gene regulatory networks (GRNs). It combines high-resolution spatio-temporal profiling of regulatory genes, strategies to perturb gene expression and quantification of perturbation effects on other genes of the network. The map of the GRN emerges by integration of these data sources and explains developmental events in terms of functional linkages between regulatory genes. This protocol has been successfully applied to regulatory processes in the sea urchin embryo, but it is generally applicable to any developmental process that relies primarily on transcriptional regulation. Unraveling the GRN for a whole tissue or organ is a challenging undertaking and, depending on the complexity, may take anywhere from months to years to complete.
详细资料
- 文献种类:期刊
- 期刊名称: Nature Protocols
- 期刊缩写: Nat Protoc
- 期卷页: 2008年第3卷第12期 1876-1887页
- ISBN: 1754-2189
相关链接 DOI URL
附件
A protocol for unraveling gene regulatory networks

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