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Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein

miaomiao 添加于 2009-11-26 22:07 | 1668 次阅读 | 0 个评论

作者
Li GC, Li N, Zhang YH, Li X, Wang YG, Liu XY, Qian WB, Liu XC
摘要
Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development.
详细资料
- 关键词: Agglutinins/*diagnostic use/genetics; Cell Line, Tumor; Humans; Leukemia, Myeloid/diagnosis/*pathology; Mannose/*metabolism; Mannose-Binding Lectin/*diagnostic use; Pinellia/genetics; Receptors, Virus/genetics; Recombinant Fusion Proteins/diagnostic use
- 文献种类:期刊
- 期刊名称: International Journal of Hematology
- 期刊缩写: Int J Hematol
- 期卷页: 2009年第89卷第5期 611-617页
- 地址: Xin Yuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China
- ISBN: 1865-3774
标签

leukemia
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