[Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells]
javegod 添加于 2010-1-3 21:06
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作 者
Liu G, Lu G, Fu J, Liu S, Xing X, Li L 摘 要
OBJECTIVE: To clone a novel gene which is related to human testis spermatogenesis apoptosis. METHODS: To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed. RESULTS: The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method. CONCLUSION: A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.-
详细资料
- 关键词: Amino Acid Sequence; Animals; Apoptosis/*genetics; Base Sequence; Cloning, Molecular; DNA, Complementary/chemistry/genetics; Female; Gene Expression; Heat-Shock Proteins; Humans; Male; Mice; Molecular Sequence Data; Proteins/*genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Spermatocytes/cytology/*metabolism
- 文献种类:期刊
- 期刊名称: Zhonghua yi xue yi Chuan xue za zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics
- 期刊缩写: Zhonghua Yi Xue Yi Chuan Xue Za Zhi
- 期卷页: 2003年 第20卷 第2期 107-110页
- 地址: Human Reproductive Engineering Institute, Xiangya Medical School, Central South University, Changsha, Hunan, 410078 P. R. China. lgxdiredtor@sina.com
- ISBN: 1003-9406
- 备注:PMID:12673577
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