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[Construction and expression of APP(SWE) transgenic mice model]

javegod 添加于 2010-1-3 21:18 | 2190 次阅读 | 0 个评论

作者
Liu W, Lu G
摘要
OBJECTIVE: To prepare transgenic mice model of human Swedish mutation of amyloid precursor protein(APP(SWE)) gene. METHODS: By pronuclear microinjection, human APP (SWE) genes were microinjected into pronuclei of zygotes of C57 and Kunming white mice. Integral zygotes were implanted into the oviducts of pseudopregnant female mice. The integration and expression of exogeneous genes in offsprings were detected by polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH) and reverse transcription-ploymerase chain reaction (RT-PCR) analysis. RESULTS: The success rate of microinjection and the birth rate were 76.62% and 10.38%, respectively. There were 6 founders and the integration rate was 35.29%. The exogeneous gene was transferred to the third generation. Expression of human APP(SWE) gene was detected in the brain, heart and muscle but not detected in the liver and kidney of transgenic mice. CONCLUSION: The transgenic mice model of human APP(SWE) gene has been prepared successfully.
详细资料
- 关键词: Amyloid beta-Protein Precursor/*genetics; Animals; Brain/metabolism; Cloning, Molecular; Female; Gene Expression; Humans; In Situ Hybridization, Fluorescence; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Muscles/metabolism; Myocardium/metabolism; Plasmids/genetics
- 文献种类:期刊
- 期刊名称: Zhonghua yi xue yi Chuan xue za zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics
- 期刊缩写: Zhonghua Yi Xue Yi Chuan Xue Za Zhi
- 期卷页: 2002年第19卷第2期 104-107页
- 地址: Human Reproductive Engineering Laboratory, Xiangya Medical College, Central-South University, Changsha, Hunan, 410078 P. R. China. zhuyanszzx@sina.com
- ISBN: 1003-9406
- 备注:PMID:11941582
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