新科学想法 › 文献管理 › 浏览文献

Relationship between microdeletion on Y chromosome and patients with idiopathic azoospermia and severe oligozoospermia in the Chinese

javegod 添加于 2010-1-3 21:24 | 1979 次阅读 | 0 个评论

作者
Fu J, Li L, Lu G
摘要
OBJECTIVES: To evaluate the relationship between microdeletion or mutation on the Y chromosome and Chinese patients with idiopathic azoospermia and severe oligozoospermia and to establish a molecular detection method. METHODS: Microdeletion or mutation detection at the AZFa (sY84 and USP9Y), AZFb, AZFc/DAZ and SRY regions of the Y chromosome. Seventy-three azoospermia and 28 severe oligozoospermia patients were evaluated using PCR and PCR-SSCP techniques. RESULTS: Twelve of 101 patients (12%) with the AZFc/DAZ microdeletion were found, including 8 with azoospermia (11%) and 4 with severe oligozoospermia (14.3%), and 1 patient had a AZFb and AZFc/DAZ double deletion. No deletions in the AZFa or SRY regions were found. No deletions in AZFa, AZFb, AZFc/DAZ or SRY regions were found in 60 normal men who had produced one or more children. CONCLUSIONS: Microdeletion on the Y chromosome, especially at its AZFc/DAZ regions, may be a major cause of azoospermia and severe oligozoospermia leading to male infertility in China. It is recommended that patients have genetic counseling and microdeletion detection on the Y chromosome before intracytoplasmic sperm injection.
详细资料
- 关键词: *Chromosome Deletion; Humans; Male; Oligospermia/*genetics; Sperm Injections, Intracytoplasmic; *Y Chromosome
- 文献种类:期刊
- 期刊名称: Chinese Medical Journal
- 期刊缩写: Chin Med J (Engl)
- 期卷页: 2002年第115卷第1期 72-75页
- 地址: Laboratory of Human Reproductive Engineering, Central South University Xiangya Medical College, Changsha 410078, China. junjiang@public.cs.hn.cn
- ISBN: 0366-6999
- 备注:PMID:11930664
标签

guangxiu lu
相关链接 URL