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[Fluorescence in situ hybridization study on the chromosome 17 aneuploidy of human oocytes failing to fertilize in vitro]

javegod 添加于 2010-1-3 21:25 | 2061 次阅读 | 0 个评论

作者
Zhang Q, Lu G
摘要
OBJECTIVE: Compare two methods of preparing human unfertilized oocytes interphase nucleus and analyze the relationship between the different in vitro fertilization(IVF) indications, ovarian stimulation protocols, women's age and frequency of chromosome 17 aneuploidy. METHODS: Tarkowski's air-drying method(3:1 methanol:acetic acid) and Coonen's 0.1% Tween 20/0.01 mol/L HCl method were used to fix human unfertilized oocytes interphase nucleus, and telomeric probe of 17qter was used by standard fluorescence in situ hybridization(FISH) procedures to confirm chromosome 17qter aneuploidy. RESULTS: Of 36 human unfertilized oocytes, 24 were haploid (66.7%), 7 were disomic (19.44%), 5 were trisomic (13.89%). The overall frequency of aneuploidy was 33.3%. There were no differences between the protocols characterized by different maternal age, IVF indication, ovarian stimulation. CONCLUSION: Tarkowski's air dry method is as good as the method of Coonen's, but the latter method can avoid the smell pollution of the methanol and acetic acid, and it is easy to operate. The chromosome 17 aneuploidy is one of the factors to cause in vitro fertilization failure of human oocytes.
详细资料
- 关键词: Adult; *Aneuploidy; Cell Nucleus/genetics/ultrastructure; Chromosomes, Human, Pair 17/*genetics/ultrastructure; DNA Probes; Diploidy; Embryo Transfer; Female; Fertilization in Vitro/methods; Haploidy; Humans; In Situ Hybridization, Fluorescence/*methods; Interphase/genetics; Karyotyping; Microscopy, Fluorescence/methods; Ovulation Induction/methods; Ovum/*physiology; Trisomy
- 文献种类:期刊
- 期刊名称: Zhonghua yi xue yi Chuan xue za zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics
- 期刊缩写: Zhonghua Yi Xue Yi Chuan Xue Za Zhi
- 期卷页: 2002年第19卷第1期 72-75页
- 地址: Human Reproductive Engineering Laboratory, Xiangya Medical College, Central-South University, Changsha, Hunan, 410078 P. R. China. lgxdirector@sina.com
- ISBN: 1003-9406
- 备注:PMID:11836694
标签

guangxiu lu
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