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An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles

gong329 添加于 2010-1-11 11:44 | 1342 次阅读 | 0 个评论

作者
Santi L, Batchelor L, Huang Z, Hjelm B, Kilbourne J, Arntzen CJ, Chen Q, Mason HS
摘要
Virus-like particles (VLPs) derived from enteric pathogens like Norwalk virus (NV) are well suited to study oral immunization. We previously described stable transgenic plants that accumulate recombinant NV-like particles (rNVs) that were orally immunogenic in mice and humans. The transgenic approach suffers from long generation time and modest level of antigen accumulation. We now overcome these constraints with an efficient tobacco mosaic virus (TMV)-derived transient expression system using leaves of Nicotiana benthamiana. We produced properly assembled rNV at 0.8 mg/g leaf 12 days post-infection (dpi). Oral immunization of CD1 mice with 100 or 250 microg/dose of partially purified rNV elicited systemic and mucosal immune responses. We conclude that the plant viral transient expression system provides a robust research tool to generate abundant quantities of rNV as enriched, concentrated VLP preparations that are orally immunogenic.
详细资料
- 关键词: Animals; Caliciviridae Infections/*prevention & control; Female; Gastroenteritis/*prevention & control; Immunoglobulin A/analysis; Intestines/immunology; Mice; Microscopy, Electron, Transmission; Norwalk virus/genetics/*immunology; Tobacco/genetics/*immunology; Tobacco Mosaic Virus/*genetics; Vaccines, Edible/genetics/immunology; Vaccines, Virosome/genetics/immunology; Viral Vaccines/genetics/*immunology; Virosomes/ultrastructure
- 文献种类:期刊
- 期刊名称: Vaccine
- 期刊缩写: Vaccine
- 期卷页: 2008年第26卷第15期 1846-1854页
- 地址: The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401, USA
- ISBN: 0264-410X
- 备注:PMID:18325641
标签

norovirus,疫苗,诺如病毒
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