Oxidative stress decreases klotho expression in a mouse kidney cell line
txq01001 添加于 2010-2-20 17:55
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作 者
Mitobe M, Yoshida T, Sugiura H, Shirota S, Tsuchiya K, Nihei H 摘 要
BACKGROUND/AIMS: Defects in klotho gene expression in the mouse result in a syndrome that resembles human aging. We recently identified expression of klotho in a mouse inner medullary collecting duct (mIMCD3) cell line for the first time, and in the present study we explored the physiological relevance of the regulation of klotho expression in the presence of oxidant stress injury. METHODS: Klotho expression was analyzed by real-time PCR, Western blot, and immuocytochemical staining during exposure to hydrogen peroxide (H2O2). Overexpression of the klotho gene was induced by klotho adenoviruses, and the number of apoptotic cells was counted by flowcytometry. RESULTS: Oxidant stress injury by H2O2 dose-dependently reduced klotho expression and diminished klotho staining. There were fewer apoptotic cells among the klotho-transfected cells than among the control cells. CONCLUSION: Klotho is expressed in cell line mIMCD3, and the klotho gene may be involved in the process of oxidative stress injury and apoptosis in this cell line.-
详细资料
- 关键词: Animals; Apoptosis/drug effects/physiology; Blotting, Western; Cell Line; Computer Systems; Dose-Response Relationship, Drug; Down-Regulation/drug effects; Gene Expression Regulation; Gene Transfer Techniques; Glucuronidase; Hydrogen Peroxide/administration & dosage/pharmacology; Immunohistochemistry/methods; *Kidney Medulla; Kidney Tubules, Collecting/cytology/*metabolism/physiology; Membrane Proteins/genetics/*metabolism/pharmacology; Mice; Mice, Inbred BALB C; Oxidants/administration &
- 文献种类:期刊
- 期刊名称: Nephron. Experimental Nephrology
- 期刊缩写: Nephron Exp Nephrol
- 期卷页: 2005年 第101卷 第2期 e67-74页
- 地址: Department of Medicine IV, Tokyo Women's Medical University, Tokyo, Japan
- ISBN: 1660-2110
- 备注:PMID:15976510
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