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有读书笔记A dynamic gene expression atlas covering the entire life cycle of rice

热1 insidi 添加于 2010-4-9 13:07 | 3836 次阅读 | 0 个评论

  •  作 者

    Wang L, Xie W, Chen Y, Tang W, Yang J, Ye R, Liu L, Lin Y, Xu C, Xiao J, Zhang Q

  •  摘 要

    Summary Growth and development of a plant are controlled by programmed expression of suits of genes at the appropriate time, tissue and abundance. Although genomic resources have been developed rapidly in recent years in rice, a model plant for cereal genome research, data of gene expression profiling are still insufficient to relate the developmental processes to transcriptomes, leaving a large gap between the genome sequence and phenotype. In this study, we generated genome-wide expression data by hybridizing 190 Affymetrix GeneChip Rice Genome Arrays with RNA from 39 tissues collected throughout the life cycle of the rice plant from two varieties, Zhenshan 97 and Minghui 63. Analyses of the global transcriptomes revealed many interesting features of dynamic patterns of gene expression across the tissues and stages. In total, 38 793 probe sets were detected as expressed and 69% of the expressed transcripts showed significantly variable expression levels among tissues/organs. We found that similarity of transcriptomes among organs corresponded well to their developmental relatedness. About 5.2% of the expressed transcripts showed tissue-specific expression in one or both varieties and 22.7% of the transcripts exhibited constitutive expression including 19 genes with high and stable expression in all the tissues. This dataset provided a versatile resource for plant genomic research, which can be used for associating the transcriptomes to the developmental processes, understanding the regulatory network of these processes, tracing the expression profile of individual genes and identifying reference genes for quantitative expression analyses.

  •  详细资料

    • 文献种类:期刊
    • 期刊名称: The Plant Journal : for Cell and Molecular Biology
    • 期刊缩写: Plant J
    • 期卷页: 2009年
    • 地址: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China
    • ISBN: 0960-7412
    • 备注:PMID:20003165

  •  标 签

    seminar 

  • 相关链接 DOI URL 

  •  insidi 的文献笔记  订阅

    【原创】

    本文是张启发院士课题组新发的一篇关于基因芯片的文章。技术创新谈不上，属于数据型的文章。

          植物特定组织、特定时间的生长发育是由一系列程序化的表达基因控制的。尽管近年来水稻作为作物中的模式作物，其基因组资源增长很快，但是对分析转录组学发育进程的相关基因表达谱数据仍然显得不足。本研究中，作者利用190昂飞基因芯片分析仪，针对来自水稻两个品种珍汕97、明恢63覆盖全部发育时期的33个组织的RNA杂交，获得了全基因表达数据。

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