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Expression of a putative alfalfa helicase increases tolerance to abiotic stress in Arabidopsis by enhancing the capacities for ROS scavenging and osmotic adjustment

热2 libingbing 添加于 2010-4-14 21:31 | 2307 次阅读 | 1 个评论

作者
Luo Y, Liu YB, Dong YX, Gao X-Q, Zhang XS
摘要
Plant helicasesareknowntobeinvolvedinsalinityandlow-temperaturetolerance. However,afunctionalinvolvementofhelicasesintheantioxidativeresponseof plants hasnotbeendescribed.WehaveisolatedaDEAD-box-containingcDNA sequence from Medicago sativa (alfalfa) thatisahomologofthepeaDNAhelicase45 (PDH45) andnamedit M. sativa helicase 1(MH1). Transienttransfectionof 35S::MH1-GFP to onionepidermisrevealedthat MH1 was localizedinthenucleus. Expression of MH1 was detectedinroots,stemsandleavesofalfalfa.Furthermore, real-time PCRanalysisrevealedthatmannitol,NaCl,methylviologenandabscisic acid inducedtheexpressionof MH1. Theectopicexpressionof MH1 in Arabidopsis improved seedgerminationandplantgrowthunderdrought,saltandoxidative stress. Thecapacityforosmoticadjustment,superoxidedismutase(SOD)and ascorbate peroxidase(APX)activitiesandprolinecontentwerealsoelevatedinthe transgenic Arabidopsis plants. Ourresultssuggestthat MH1 responds toreactive oxygen species(ROS)andfunctionsindroughtandsaltstresstolerancebyenhancing the capacitiesforROSscavengingandosmoticadjustment. & 2008 ElsevierGmbH.Allrightsreserved.
详细资料
- 文献种类:期刊
- 期刊名称: Journal of Plant Physiology
- 期刊缩写: Journal of Plant Physiology
- 期卷页: 2009年第166卷第4期 385-394页
- ISBN: 0176-1617
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附件
Expression of a putative alfalfa helicase increases tolerance to abiotic stress in Arabidopsis by enhancing the capacities for ROS scavenging and osmo

libingbing 的文献笔记订阅

【原创】这篇文章从苜蓿中克隆得到了一个解旋酶-MH1，做了序列进化分析，亚细胞定位分析说明这个基因的特点，通过realtime PCR进行了四种非生物胁迫下的表达特性分析，发现全都明显上调表达，最后对拟南芥进行了超量表达，对转基因拟南芥做了抗逆性分析，发现提高了在干旱，盐，和氧化胁迫下的抗性，进一步分析机理，作者根据测定sod和apx活性发现上升，而钠离子并未发生明显变化推测MH1基因提高了抗逆性原因可能主要是提高清除ROS能力，从而提高对逆境的适应性，而不是常认为的那样减少细胞内钠离子的浓度，这个推测比较有创新性，但就像作者最后说得那样，还需要进一步实验证明，另外，现在有很多人认为豆科来源的基因用模式植物拟南芥验证不一定准确，最好还能回归豆科植物来验证。

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