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Overexpression of a mitochondrial ATP synthase small subunit gene (AtMtATP6) confers tolerance to several abiotic stresses in Saccharomyces cerevisiae and Arabidopsis thaliana

热2 libingbing 添加于 2010-4-15 03:58 | 2427 次阅读 | 2 个评论

作者
Zhang X, Liu S, Takano T
摘要
Mitochondrial F1F0-ATPase is a key enzyme in plant metabolism, providing cells with ATP that uses the transmembrane electrochemical proton gradient to drive synthesis of ATP. A 6 kDa protein (At3g46430) has been previously purified from Arabidopsis thaliana mitochondrial F1F0-ATPase. In this study, the gene (AtMtATP6; GenBank accession no. AK117680) encoding this protein was isolated from Arabidopsis and characterized. Northern blot analyses showed that the expression of AtMtATP6 gene in Arabidopsis suspension-cultured cells was induced by several abiotic stresses from salts, drought, and cold. Over-expression of AtMtATP6 gene in transgenic yeast and Arabidopsis plants increased the resistance to salts, drought, oxidative and cold stresses. Taken together, our data raise the possibility that induction of the F1F0- ATPase plays a role in stress tolerance.
详细资料
- 关键词: Arabidopsis/cytology/genetics/*metabolism; Arabidopsis Proteins/*biosynthesis/genetics; Cold Temperature; Membrane Proteins/*biosynthesis/genetics; Mitochondrial Proteins/*biosynthesis/genetics; Proton-Translocating ATPases/genetics/*metabolism; Recombinant Proteins/*biosynthesis/genetics; Saccharomyces cerevisiae/genetics/*metabolism; Salts/pharmacology
- 文献种类:期刊
- 期刊名称: Biotechnology Letters
- 期刊缩写: Biotechnol Lett
- 期卷页: 2008年第30卷第7期 1289-1294页
- 地址: Asian Natural Environment Science Center, The University of Tokyo, 1-1-1 Midori-cho, Nishitokyo City, Tokyo, 188-0002, Japan
- ISBN: 0141-5492
- 备注:PMID:18338219
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附件
ATP酶提高了抗逆性.pdf

libingbing 的文献笔记订阅

【原创】本文从拟南芥克隆得到了一个线粒体F1F0-ATPase，该酶在参与植物生长代谢中起到关键作用，进年来有相应文献认为在非生物胁迫中也起到重要作用，本文就是其中一篇。通过对转基因拟南芥悬浮细胞在几种非生物胁迫下的表达特性分析（northernblot)，另外通过转化酵母及拟南芥进行胁迫下抗性分析，发现提高了抗逆性。总体来说，这篇论文只是通过这几个实验发现了一种现象，但就其本质并未深入的进行研究，如既然是一个ATPase，至少要研究一下这个酶的活性有没有改变，而且用拟南芥表型证据也不是很充分，只作了萌发期的，萌发期实验是非常受种子活力影响的，最好加上幼苗期以及成苗期的实验证据。

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