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The Arabidopsis SLEEPY1 Gene Encodes a Putative F-Box Subunit of an SCF E3 Ubiquitin Ligase

热2 cflikunlun 添加于 2010-4-20 17:40 | 2679 次阅读 | 2 个评论

作者
McGinnis KM
摘要
The Arabidopsis SLY1 ( SLEEPY1 ) gene positively regulates gibberellin (GA) signaling. Positional cloning of SLY1 revealed that it encodes a putative F-box protein. This result suggests that SLY1 is the F-box subunit of an SCF E3 ubiquitin ligase that regulates GA responses. The DELLA domain protein RGA (repressor of ga1-3 ) is a repressor of GA response that appears to undergo GA-stimulated protein degradation. RGA is a potential substrate of SLY1, because sly1 mutations cause a significant increase in RGA protein accumulation even after GA treatment. This result suggests SCF SLY1 -targeted degradation of RGA through the 26S proteasome pathway. Further support for this model is provided by the observation that an rga null allele partially suppresses the sly1-10 mutant phenotype. The predicted SLY1 amino acid sequence is highly conserved among plants, indicating a key role in GA response.
详细资料
- 文献种类:期刊
- 期刊名称: THE PLANT CELL ONLINE
- 期卷页: 第15卷第5期 1120-1130页
- ISBN: 1040-4651
标签

GA signal
相关链接 DOI URL

cflikunlun 的文献笔记订阅

【原创】这篇文章克隆了sly1基因，进行了表达，对表达的蛋白进行了结构分析，发现该基因是SCFE3ubiquitin ligase 的一个F-box的亚基，并且通过回复突变等证明RGA是该基因编码的酶的底物，并且推测了该基因在GA信号通路中的作用，文章的结论在现在看来都似乎已经成为关于GA信号通路中成型的“结论”了。

另外，这是我第一次看见实际应用图位克隆，真的是很麻烦！

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