Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana

【原创】本文通过miRNA 芯片获得相应盐，干旱，冷胁迫的miRNA（14个），基于它们靶基因地特点，分为三类，eight miRNAs—miR156, miR159, miR165, miR169, miR171, miR172,miR319, and miR396—which target transcription factors，再进一步响应胁迫；Thesecond category includes miR167, miR168, miR393, and
miR394, which are related to direct response to stresses ，The last category includes miR397 and miR408, whose target genes are hydrolase and oxidoreductase coding genes, which respond to many
stresses ，对于未检测到的miRNA，作者分析了原因，对于已经报道明显响应胁迫的miR389 and miR400 ，可能是treatment methods or technical differences between RNA gel blot and microarrayanalysis. 其他的，perhaps at low levels at this stage or their expression is limited to specific cell types or particular growth conditions；通过sqRTPCR来验证芯片结果，除了miR156h and miR167d，符合芯片结果；作者提取20个miRNA上游1000bp序列通过PLANTCARE分析了miRNA的启动子发现存在胁迫响应顺式作用元件，进一步证明了之前的14个miRNA可能参与逆境响应。对其他物种的miRNA的研究有参考价值！