下文献，学文献

dr.qinan · 发表于 2009-5-21 19:06:16

文献是科研的一大要素，习惯性地下载文献，粗略地浏览文献，似乎所得不是很多，或许是缺少一个学文献的氛围吧。
借着这个板块，尝试着监督自己，也希望起个抛砖引玉的作用，和大家一起学文献，探讨文献，并慢慢养成习惯。

dr.qinan · 发表于 2009-5-22 23:19:22

D1:
Inhibition of osteoblastic bone formation by nuclear factor-kB
This paper from nature medicine has presented us with plenty of logical evidence to demonstrate the important role of NF-kB in osteoblast, whose function is upregulated by inhibition of NF-kB in osteoblasts.
The structure of this paper is worth of learning.
step 1: by the construction of conditional NF-kB knockout mice, the authors verified the specific knockdown by southern blot, RT-PCR and western blot. Based on these results, they employed regular microCT and hisotological methods to demonstrate that the bone mineral density and bone volume is increased in these conditional knockout mice, which is due to the strengthened osteoblastic function, but not osteoclasts.
Then, they look into the signaling pathway involving NF-kB and confirmed this role of NF-kB in TNF induced phosphoration of degration of IKBalpha and phophoration of P65.What's more ,they verified this effect by rescue experiment by overexpressing p65 to activate NF-KB.
Then, they used OVX mice model to further confirm the role of NF-kB in osteoblasts during osteoporosis.
Finally, the author explored the molecular mechanism by analysis many involved pathways and find out the c-JUN and JNK were activated. again, they used RNAi to further confirm this conclusion.
In conclusion ,the logical analysis is worthy of learning.

dr.qinan · 发表于 2009-5-24 18:56:55

D2: Giant osteoclast formation and long-term oral bisphosphonate therapy
This paper published in New England is a well designed, randomized paper that helps us the understanding How bisphosphonates decrease bone resorption as the anti-osteoporosis methods.
In addtion to the delicate clinical design, the most interesting part is that the authors found that giant osteoclasts that detached from bone may be disabled to function for bone resorption, which is quite different from traditional understanding of osteoclasts.

dr.qinan · 发表于 2009-5-24 19:01:32

尽管只做了两天，但觉得这种学习文献的方法对自己很有好处：
一、锻炼英文：尝试着用英文写summary，这样可以慢慢锻炼自己的写作能力。记得昨天看到的那篇Nature上关于如何做一个优秀的PhD的文章中，就提到了这点。一定要提高英文写作能力，从而使你在总结自己科研工作的时候得心应手。这点，我还有好长的路要走。如果大家有兴趣，不妨给自己建立个帖子，一起用英文来总结，学习文献。当然，你也可以在这个帖子中跟贴，我们还可以互相学习文献，同时像在太傻上一样，互相帮助修改文法。
二、整理文章：写了summary之后，对这篇文章的整体有了更深入的了解，同时还便于以后记忆。一举多的。
大家一起来，尝试着写文献的summary吧。