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有读书笔记Development of Autoantibody Signatures as Novel Diagnostic Biomarkers of Non-Small Cell Lung Cancer

ymmffp 添加于 2010-7-14 21:48 | 1392 次阅读 | 0 个评论

  •  作 者

    Wu LL, Chang WJ, Zhao J, Yu YW, Tan X, Su T, Zhao L, Huang S, Liu S, Cao G

  •  摘 要

    PURPOSE: To select autoantibody signatures as noninvasive biomarkers of non-small cell lung cancer (NSCLC).EXPERIMENTAL DESIGN: A phage cDNA expression library was constructed with fresh samples from 30 lung cancer patients and biopanned using serum pools of 10 NSCLC patients and 10 healthy controls. A 6-phage-peptide detector was discovered by two-step immunoscreenings, and validated in an independent set of 90 NSCLC patients and 90 matched healthy controls, 30 NSCLC patients with chemotherapy, and 12 chronic obstructive pulmonary disease (COPD) patients. Expression of a peptide target was validated by using immunohistochemistry. Factors affecting NSCLC-related death were evaluated by Cox regression analysis. RESULTS: Six phage-peptide clones showing higher seroreactivity than others in 30 NSCLC patients were selected for diagnostic validation. The 6-phage-peptide detector was able to discriminate between NSCLC patients and healthy controls with a sensitivity and specificity of greater than 92%, and had similar validity for indicating NSCLC at early stage. The seroreactivity of the 6 phage-peptides was significantly higher in the NSCLC patients than in those with chemotherapy and the COPD patients, respectively. Of the 6 phage-peptides, one encoded a peptide showing 100% homology to olfactomedin 1. Expression of olfactomedin 1 protein was significantly higher in lung adenocarcinoma than in lung cancer of other histological types and normal lung tissues. The autoantibody signature was not associated with the prognosis of the NSCLC patients. CONCLUSIONS: The 6-phage-peptide-detector stands out as promising diagnostic biomarkers for NSCLC, unlikely for NSCLC relapse after chemotherapy. Olfactomedin 1 may be a novel target of lung adenocarcinoma.

  •  详细资料

    • 文献种类: Journal Article
    • 期刊名称: Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
    • 期刊缩写: Clin Cancer Res
    • 期卷页: 2010年
    • 地址: Epidemiology, Second Military Medical University
    • ISBN: 1078-0432
    • 备注:PMID:20501620

  • 学科领域 生物医药 » 基础医学

  •  标 签

    噬菌体展示  非小细胞肺癌 

  • 相关链接 DOI URL 

  •  ymmffp 的文献笔记  订阅

    自身抗体作为非小细胞肺癌新诊断标志物的研究

    实验思路：利用30个肺癌患者标本构建噬菌体表达文库，用10个NSCLC患者及10个正常人血清进行生物淘洗。两步免疫筛选后获得了6个噬菌体肽。在90个NSCLC患者及90个正常人中用免疫组化方法验证6个噬菌体肽。30个NSCLC患者进行了化疗，12个慢性阻塞性肺疾病（COPD）。用Cox回归分析方法进行NSCLC死亡相关因素的分析。

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