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A model of yeast cell-cycle regulation based on multisite phosphorylation

xbinbzy 添加于 2010-8-30 22:02 | 2679 次阅读 | 0 个评论

作者
Barik D, Baumann WT, Paul MR, Novak B, Tyson JJ
摘要
In order for the cell's genome to be passed intact from one generation to the next, the events of the cell cycle (DNA replication, mitosis, cell division) must be executed in the correct order, despite the considerable molecular noise inherent in any protein-based regulatory system residing in the small confines of a eukaryotic cell. To assess the effects of molecular fluctuations on cell-cycle progression in budding yeast cells, we have constructed a new model of the regulation of Cln- and Clb-dependent kinases, based on multisite phosphorylation of their target proteins and on positive and negative feedback loops involving the kinases themselves. To account for the significant role of noise in the transcription and translation steps of gene expression, the model includes mRNAs as well as proteins. The model equations are simulated deterministically and stochastically to reveal the bistable switching behavior on which proper cell-cycle progression depends and to show that this behavior is robust to the level of molecular noise expected in yeast-sized cells (~50 fL volume). The model gives a quantitatively accurate account of the variability observed in the G1-S transition in budding yeast, which is governed by an underlying sizer+timer control system.
详细资料
- 文献种类: Journal Article
- 期刊名称: Molecular Systems Biology
- 期刊缩写: Mol Syst Biol
- 期卷页: 2010年第6卷
- ISBN: 1744-4292
学科领域生物医药 » 中医中药学
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