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An Orange Ripening Mutant Links Plastid NAD (P) H Dehydrogenase Complex Activity to Central and Specialized Metabolism during Tomato Fruit Maturation

dfhfzyl 添加于 2010-12-10 11:20 | 2401 次阅读 | 0 个评论

作者
ShaiNashilevitz
摘要
In higher plants, the plastidial NADH dehydrogenase (Ndh) complex supports nonphotochemical electron ﬂuxes from stromal electron donors to plastoquinones. Ndh functions in chloroplasts are not clearly established; however, its activity was linked to the prevention of the overreduction of stroma, especially under stress conditions. Here, we show by the characterization of OrrDs, a dominant transposon-tagged tomato (Solanum lycopersicum) mutant deﬁcient in the NDH-M subunit, that this complex is also essential for the fruit ripening process. Alteration to the NDH complex in fruit changed the climacteric, ripening-associated metabolites and transcripts as well as fruit shelf life. Metabolic processes in chromoplasts of ripening tomato fruit were affected in OrrDs, as mutant fruit were yellow-orange and accumulated substantially less total carotenoids, mainly b-carotene and lutein. The changes in carotenoids were largely inﬂuenced by environmental conditions and accompanied by modiﬁcations in levels of other fruit antioxidants, namely, ﬂavonoids and tocopherols. In contrast with the pigmentation phenotype in mature mutant fruit, OrrDs leaves and green fruits did not display a visible phenotype but exhibited reduced Ndh complex quantity and activity. This study therefore paves the way for further studies on the role of electron transport and redox reactions in the regulation of fruit ripening and its associated metabolism.
详细资料
- 文献种类: Journal Article
- 期刊名称: plant cell
学科领域农业科学 » 农学
所属群组
citrus-pigment
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An Orange Ripening Mutant Links Plastid NAD (P) H Dehydrogenase Complex Activity to Central and Specialized Metabolism during Tomato Fruit Maturation
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An Orange Ripening Mutant Links Plastid NAD (P) H Dehydrogenase Complex Activity to Central and Spec