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Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity

cyzhgt 添加于 2011-9-9 09:17 | 1327 次阅读 | 0 个评论

作者
Vallee-Belisle A, Bonham AJ, Reich NO, Ricci F, Plaxco KW
摘要
The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-kappaB), and measure binding activity directly in crude nuclear extracts.
详细资料
- 文献种类: Journal Article
- 期刊名称: Journal of the American Chemical Society
- 期刊缩写: J Am Chem Soc
- 期卷页: 2011年第133卷第35期 13836-13839页
- 地址: Department of Chemistry and Biochemistry, section signInterdepartmental Program in Biomolecular Science and Engineering, University of California , Santa Barbara, California 93106, United States
- ISBN: 0002-7863
- 备注:PMID:21815647
学科领域生物医药 » 生物学
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