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Effect of miR-451 on the Biological Behavior of the Esophageal Carcinoma Cell Line EC9706

icecream 添加于 2012-10-14 09:00 | 1144 次阅读 | 0 个评论

作者
Wang T, Zang W-Q, Li M, Wang N, Zheng Y-L, Zhao G-Q
摘要
BACKGROUND: MicroRNAs play important roles in coordinating a variety of cellular processes. Abnormal expression of miRNAs has been linked to several cancers. However, the functional role of miR-451 in esophageal squamous cell carcinoma remains unclear. AIMS: The present study explored the effects of miR-451 on the biological behavior of the esophageal carcinoma cell line EC9706. METHODS: Synthetic miR-451 mimics were transfected into EC9706 cells using Lipofectamine 2000. The expression of miR-451 was analyzed by RT-PCR and the expressions of Bcl-2, AKT and phosphorylated AKT were analyzed by Western blotting. The MTT assay, soft agar colony formation assay, transwell assay and FACS were used to assess the effect of miR-451 on EC9706 cell proliferation, invasion, metastasis and apoptosis. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice. RESULTS: In comparison to the controls, a significant increase in the expression of miR-451 was associated with significantly decreased expressions of Bcl-2, AKT and p-AKT, and a significant increase in the apoptosis rate. The number of cell clones was significantly decreased by miR-451 expression, which also caused the inhibition of cell proliferation. The average number of cells penetrating the matrigel was significantly lower than the controls. Injection of miR-451 inhibited tumor growth in a xenograft model. CONCLUSIONS: Upregulated expression of miR-451 induced apoptosis and suppressed cell proliferation, invasion and metastasis in the esophageal carcinoma cell line EC9706. In addition, injection of miR-451 inhibited tumor growth in a xenograft model of esophageal cancer.
详细资料
- 文献种类: Journal Article
- 期刊名称: Digestive Diseases and Sciences
- 期刊缩写: Dig Dis Sci
- 期卷页: 2012年
- 地址: Department of Tumor, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China, wang37127070@tom.com
- ISBN: 0163-2116
学科领域生物医药 » 预防卫生学
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