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High expression of Lin28 is associated with tumour aggressiveness and poor prognosis of patients in oesophagus cancer

icecream 添加于 2012-10-14 23:28 | 1181 次阅读 | 0 个评论

作者
Hamano R, Miyata H, Yamasaki M, Sugimura K, Tanaka K, Kurokawa Y, Nakajima K, Takiguchi S, Fujiwara Y, Mori M, Doki Y
摘要
BACKGROUND: Lin28 is a negative regulator of the tumour suppressor microRNA, let-7, suggesting its role in tumourigenesis. However, the clinical significance of Lin28 expression in oesophageal cancer has not been elucidated. METHODS: Lin28 and Lin28B expression was examined by immunohistochemistry in 161 tissues from patients with oesophageal cancer who had undergone curative surgery. The relationship between the expressions of Lin28 and Lin28B and various clinicopathological factors was examined. In vitro assays were conducted to determine the role of Lin28 in aggressiveness of oesophageal cancers using oesophageal cancer cell line. RESULTS: Lin28 and Lin28B were overexpressed in oesophageal cancer cells compared with non-cancerous epithelial cells, especially in the invasive front. High expression of Lin28 and Lin28B correlated significantly with lymph node metastasis and poor prognosis. High expression of Lin28B expression correlated significantly with low expression of let-7. Multivariate analysis also identified Lin28B expression as an independent prognostic factor. In vitro assays showed that the proliferative and invasive activities were significantly reduced in Lin28B-knockdown cells, compared with control cells. CONCLUSION: High expression of Lin28 is associated with poor prognosis and high tumour aggressiveness in oesophageal cancer and these effects are mediated through increased proliferation and invasiveness of oesophageal cancer cells.
详细资料
- 关键词: Cell Proliferation; DNA-Binding Proteins/biosynthesis/genetics/*metabolism; Esophageal Neoplasms/diagnosis/genetics/*metabolism/*pathology; Female; Humans; Immunohistochemistry; Male; Middle Aged; Prognosis; RNA-Binding Proteins/biosynthesis/genetics/*metabolism; Real-Time Polymerase Chain Reaction
- 文献种类: Journal Article
- 期刊名称: British Journal of Cancer
- 期刊缩写: Br J Cancer
- 期卷页: 2012年第106卷第8期 1415-1423页
- 地址: Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Japan
- ISBN: 0007-0920
学科领域生物医药 » 预防卫生学
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