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有读书笔记Non-resorbing osteoclasts induce migration and osteogenic differentiation of mesenchymal stem cells

热3 dr.qinan 添加于 2009-12-6 21:38 | 1485 次阅读 | 0 个评论

  •  作 者

    Kreja L, Brenner RE, Tautzenberger A, Liedert A, Friemert B, Ehrnthaller C, Huber-Lang M, Ignatius A

  •  摘 要

    Osteoclast activity has traditionally been regarded as restricted to bone resorption but there is some evidence that also non-resorbing osteoclasts might influence osteoblast activity. The aim of the present study was to further investigate the hypothesis of an anabolic function of non-resorbing osteoclasts by investigating their capability to recruit mesenchymal stem cells (MSC) and to provoke their differentiation toward the osteogenic lineage. Bone-marrow-derived human MSC were exposed to conditioned media (CM) derived from non-resorbing osteoclast cultures, which were generated from human peripheral blood monocytes. Osteogenic marker genes (transcription factor Runx2, bone sialoprotein, alkaline phosphatase (AP), and osteopontin) were significantly increased. Osteogenic differentiation (OD) was also proved by von Kossa and AP staining occurred in the same range as in MSC cultures stimulated with osteogenic supplements. Chemotactic responses of MSC were measured with a modified Boyden chamber assay. CM from osteoclast cultures induced a strong migratory response in MSC, which was greatly reduced in the presence of an anti-human platelet-derived growth factor (PDGF) receptor beta antibody. Correspondingly, significantly increased PDGF-BB concentrations were measured in the CM using a PDGF-BB immunoassay. CM derived from mononuclear cell cultures did not provoke MSC differentiation and had a significantly lower migratory effect on MSC suggesting that the effects were specifically mediated by osteoclasts. In conclusion, it can be suggested that human non-resorbing osteoclasts induce migration and OD of MSC. While effects on MSC migration might be mainly due to PDGF-BB, the factors inducing OD remain to be elucidated. J. Cell. Biochem. (c) 2009 Wiley-Liss, Inc.

  •  详细资料

    • 文献种类: Journal Article
    • 期刊名称: Journal of Cellular Biochemistry
    • 期刊缩写: J Cell Biochem
    • 期卷页: 2009年
    • 地址: Institute of Orthopaedic Research and Biomechanics, Center of Musculoskeletal Research Ulm, University of Ulm, Ulm, Germany
    • ISBN: 1097-4644
    • 备注:PMID:19950208

  • 学科领域 生物医药 » 临床医学

  •  所属群组

    骨科学  

  • 相关链接 DOI URL 

  •  dr.qinan 的文献笔记  订阅

    An interesting paper on osteclast osteblast cross talk. This paper focus on the function of non-resorption oteoclast, which can play an osteogenic role. Previously, it has been demonstrated that several factors are involved in osteoclast osteoblast cross talk. Here in this paper, the authors used conditional medium from non-resorption osteoclast and find the increase of PDGF-BB concentration in this conditional medium , which can result in osteoblast differentiation.

    An interesting paper for me.

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