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有读书笔记有附件Identification of pathways regulating cell size and cell-cycle progression by RNAi

阿平 添加于 2009-9-29 05:58 | 1987 次阅读 | 0 个评论

  •  作 者

    Bjorklund M, Taipale M, Varjosalo M, Saharinen J, Lahdenpera J, Taipale J

  •  摘 要

    Many high-throughput loss-of-function analyses of the eukaryotic cell cycle have relied on the unicellular yeast species Saccharomyces cerevisiae and Schizosaccharomyces pombe. In multicellular organisms, however, additional control mechanisms regulate the cell cycle to specify the size of the organism and its constituent organs. To identify such genes, here we analysed the effect of the loss of function of 70% of Drosophila genes (including 90% of genes conserved in human) on cell-cycle progression of S2 cells using flow cytometry. To address redundancy, we also targeted genes involved in protein phosphorylation simultaneously with their homologues. We identify genes that control cell size, cytokinesis, cell death and/or apoptosis, and the G1 and G2/M phases of the cell cycle. Classification of the genes into pathways by unsupervised hierarchical clustering on the basis of these phenotypes shows that, in addition to classical regulatory mechanisms such as Myc/Max, Cyclin/Cdk and E2F, cell-cycle progression in S2 cells is controlled by vesicular and nuclear transport proteins, COP9 signalosome activity and four extracellular-signal-regulated pathways (Wnt, p38betaMAPK, FRAP/TOR and JAK/STAT). In addition, by simultaneously analysing several phenotypes, we identify a translational regulator, eIF-3p66, that specifically affects the Cyclin/Cdk pathway activity.

  •  详细资料

    • 关键词: Active Transport, Cell Nucleus; Animals; Cell Cycle/*genetics; Cell Line; Cell Size; Conserved Sequence/genetics; Drosophila Proteins/*genetics/*metabolism; Drosophila melanogaster/*cytology/*genetics/metabolism; Gene Library; Genes, Insect/genetics; Humans; Phenotype; Phosphorylation; *RNA Interference; Signal Transduction
    • 文献种类:期刊
    • 期刊名称: Nature
    • 期刊缩写: Nature
    • 期卷页: 2006年  第439卷 第7079期 1009-1013页
    • 地址: Molecular and Cancer Biology Program, Biomedicum Helsinki, PO Box 63 (Haartmaninkatu 8), FI-00014 University of Helsinki, Finland
    • ISBN: 1476-4687
    • 备注:PMID:16496002

  •  标 签

    细胞周期 

  • 相关链接 DOI URL 

  •  附 件

    PDF附件文章  附加材料中的excel表 

  •  阿平 的文献笔记  订阅

    非常重要的原始数据，围绕这些数据，我们可以做很多细胞周期相关研究，我所关心的激酶和磷酸酶自然是细胞周期中的权利机构，需仔细研究

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