新科学想法 › 学术文库 › 学术文献 › 浏览文献

Aurora B kinase activation requires survivin priming phosphorylation by PLK1

阿平添加于 2010-12-21 07:24 | 3094 次阅读 | 0 个评论

作者
Chu Y, Yao PY, Wang W, Wang D, Wang Z, Zhang L, Huang Y, Ke Y, Ding X, Yao X
摘要
During cell division, chromosome segregation is orchestrated by the interaction of spindle microtubules with the centromere. Accurate attachment of spindle microtubules to kinetochore requires the chromosomal passenger of Aurora B kinase complex with borealin, INCENP and survivin (SUR). The current working model argues that SUR is responsible for docking Aurora B to the centromere whereas its precise role in Aurora B activation has been unclear. Here, we show that Aurora B kinase activation requires SUR priming phosphorylation at Ser20 which is catalyzed by polo-like kinase 1 (PLK1). Inhibition of PLK1 kinase activity or expression of non-phosphorylatable SUR mutant prevents Aurora B activation and correct spindle microtubule attachment. The PLK1-mediated regulation of Aurora B kinase activity was examined in real-time mitosis using fluorescence resonance energy transfer-based reporter and quantitative analysis of native Aurora B substrate phosphorylation. We reason that the PLK1-mediated priming phosphorylation is critical for orchestrating Aurora B activity in centromere which is essential for accurate chromosome segregation and faithful completion of cytokinesis.
详细资料
- 文献种类: Journal Article
- 期刊名称: Journal of Molecular Cell Biology
- 期刊缩写: J Mol Cell Biol
- 期卷页: 2010年
- 地址: Anhui Key Laboratory of Cellular Dynamics and Chemical Biology, University of Science and Technology of China, Hefei 230027, China
- ISBN: 1759-4685
- 备注:PMID:21148584
学科领域生物医药 » 生物学
所属群组
细胞周期
标签

Aurora PLk1
相关链接 DOI URL